| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1752 |
| 발표분야 |
생물화공 |
| 제목 |
Study on verification for affinity to antimicrobial peptide PG-1 of DNA aptamer in in vitro using various assay methods |
| 초록 |
Antimicrobial peptides are used in various organisms as a defense system against infection. The peptides are critical to bacteria and fungi, however have slight toxicity in mammalian and plant cells. For this reason, it is considered that antimicrobial peptides are alternative matters for defensing against microbial infection. PG-1 (Protegrin-1) is one of the antimicrobial peptide in the treatment of local or systemic infections. For expand application of PG-1 peptide, we graft the aptamers. Aptamers are consist of single-stranded DNA or RNA and they can bind to various targets with high affinity and specificity. In a previous study, we developed DNA aptamer that has specific affinity to PG-1 peptide. In this study, we proof the specific affinity between PG-1 and DNA aptamer using various assay methods. This study contribute to development of natural antimicrobial material. This work was supported by the Human Resource Training Program for Regional Innovation and Creativity through the Ministry of Education and National Research Foundation of Korea (NRF-2015H1C1A1035921). |
| 저자 |
박대영1, Phat-Loc Nguyen1, Simranjeet Singh Sekhon1, 안지영1, 민지호2, 김양훈1
|
| 소속 |
1충북대, 2전북대 |
| 키워드 |
생물화공 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
