| 초록 |
As the resistance of the currently prevalent influenza A virus (IAV) drugs has been illuminated, interest in hemagglutinin inhibition - entry blocker is increasing. In particular, sialyllactose (SL), the main residue present in the host cell receptor, has attracted attention as a candidate for the blocker. In this study, we developed a range of synthetic sialyl receptors with controlled flexibility and multivalency. Sialyllactose was coupled to lysine-based dendritic molecules via flexible linkers to impart the multivalent effect. Then, the interaction between synthetic sialyl receptors and IAV of various strains was investigated using surface plasmon resonance. We also present our effort to optimize the surface chemistry to avoid non-specific binding of the virus onto chip surface during the analysis. As a result, we could quantify the interaction between α2-3 SL and α2-6 SL receptors with various IAV strains, thereby providing useful information on designing synthetic IAV blockers. |
