| 학회 | 한국화학공학회 |
| 학술대회 | 2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 | 23권 1호, p.501 |
| 발표분야 | 생물화공 |
| 제목 | Engineering of Escherichia coli for production of native-sized spider silk protein |
| 초록 | Naturally found spider silk and elastin protein attract attention due to its outstanding physical properties coming from the highly repeated structure and size. However, the exceptional structure and size limits expression in heterologous hosts, where the repetitive sequences in mRNA create extensive secondary structures. And these structures decrease ribosome processivity and assist mRNA degradation. Using the naturally found protein, spider dragline silk protein, we present techniques to solve biological problems that occurred: using metabolic engineering as well as decreasing RNase to assist production of the protein. The newly synthesized native-size spider dragline silk protein produced increased titer than those reported previously. The results provide insight into approaches to control expression of useful recombinant proteins containing high molecular weight and highly repetitive sequence. (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557) |
| 저자 | 고유성, 정해나, Xiao-Xia Xia, 이상엽 |
| 소속 | KAIST |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
