| 학회 | 한국화학공학회 |
| 학술대회 | 2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 | 20권 1호, p.386 |
| 발표분야 | 생물화공 |
| 제목 | Development of Aptamer Based Detection Assay using Streptococcus Mutans Specific Binding Aptamers |
| 초록 | Streptococcus mutans have been associated with a primary aetiological agent of dental caries in animals and humans, and it causes tooth enamel corrosion, vulnerable to decay. The importance of S. mutans in the development of dental cares has been studied extensively. General technique known as diagnosis methods of S. mutans are based on PCR(Polymerase chain reaction) and MUTANS TEST Kit using antibody, which require multiple steps and limit detection range of below 5x105CFU/ml. Aptamers have great potential for biosensor assay development, given their small size, easy synthesis, a low cost, and high target specificity. In this study, we isolated DNA aptamer binding to S. mutans by SPR(Surface Plasmon resonance) and developed the aptamer–based detection assay such as ELISA(enzyme-immunosorbent assay). Aptamer selected by whole-cell of S. mutans show that high specificity and affinity more binding to S. mutans than to other species. Therfore, this S. mutans specific aptamer and its assay method with high specificity can be used as an alternative method for the fast and precise detection of oral disease. |
| 저자 | 이경아1, 이상희1, Simranjeet Singh Sekhon1, 민지호2, 김양훈1 |
| 소속 | 1충북대, 2전북대 |
| 키워드 | aptamer; Streptococcus mutans; aptamer-based detection assay |
| 원문파일 | 초록 보기 |
