| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
학생우수논문발표(박사과정) |
| 제목 |
Light-Triggered In Situ Biosynthesis of Artificial Melanin for Skin Protection |
| 초록 |
Tyrosinase-mediated melanin synthesis is an essential biological process that can protect skin from UV radiation and radical species. The I41Y mutant of Streptomyces avermitilis tyrosinase (SaTy) shows enzymatic activity comparable to that of wild-type SaTy. This Y41 is replaced with photocleavable o-nitrobenzyl tyrosine (ONBY) with amber codon and ONBY-tRNA synthetase/tRNA pairs. The ONBY efficiently blocks the active site and tyrosinase activity is rapidly recovered by the photo-cleavage of ONBY. The activated PaTy successfully oxidizes L-tyrosine to synthesize melanin particles. Using liposomes containing PaTy (PaTy_Lip), PaTy is transdermally delivered into ex vivo porcine skin and in vivo mouse skin tissues, thus achieving the in situ biosynthesis of artificial melanin for skin tissue protection under UV irradiation. The results of this study demonstrate that this biomimetic system can recapitulate the biosynthetic analogs of naturally occurring melanin. |
| 저자 |
이욱재, 김병기
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| 소속 |
서울대 |
| 키워드 |
artificial melanosome; photoactivatable tyrosinase; o-nitrobenzyl tyrosine; transdermal delivery; skin protection
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| E-Mail |
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