| 학회 |
한국공업화학회 |
| 학술대회 |
2005년 가을 (10/28 ~ 10/29, 건국대학교) |
| 권호 |
9권 2호 |
| 발표분야 |
정밀화학 |
| 제목 |
Optimization of protein solubility for proteomic study of Streptomyces peucetius subsp. caesius ATCC 27952 |
| 초록 |
Streptomyces peucetius produces doxorubicin which is a clinically important chemotherapeutic agent. Recently its full genome sequence has been completely sequenced. So it is needed to study protein phenotype, regulon, stimulon, proteomic signature, etc. through protein expression profile. A two-dimensional gel electrophoresis(2-DE) reveals most of a particular expression profile. Here we show the optimal condition of the protein solubilization for 2-DE to prepare 2-D reference map. In order to maximize protein solubility of Streptomyces peucetius, the optimal combination of chaotropic agents, a reducing agent, and a detergent was developed for 2-DE. |
| 저자 |
이광원1, 송은정1, 이희찬2, 류광경2, 송재경2, 김병기1
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| 소속 |
1서울대, 2선문대 |
| 키워드 |
Streptomyces peucetius; Proteomics; 2D gel electrophoresis
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| E-Mail |
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