화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터)
권호 16권 2호, p.1575
발표분야 생물화공
제목 Fabrication of protein thin film using various linkers for investigating variation of redox property
초록 Thin film composed of metalloprotein using various chemical linkers was fabricated for investigating chemical linker-length effect between gold substrate and immobilized protein. Myoglobin, it is well-known metalloprotein which has iron ions of heme group, was utilized in this research. The changes of thickness due to the immobilization were confirmed by surface Plasmon resonance spectroscopy and the morphology changes of immobilized surface were validated with atomic force microscopy. Cyclic voltammetry was utilized to confirm redox property of immobilized protein with chemical linkers having various lengths.
Acknowledgement: This research was supported by The Nano/Bio Science&Technology Program(M10536090001-05N3609-00110) of the Ministry of Education,Science and Technology(MEST), by the Original Technology Research Program for Brain Science through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology (2009-0093907), by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (2010-0000845).
저자 정용호1, 이 택1, 민준홍2, 최정우1
소속 1서강대, 2경원대
키워드 metalloprotein; SPR; AFM; CV
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