| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Synthetic biological device: engineering of the electromagnetic perceptive gene for the external control of calcium ion transport into the cell |
| 초록 |
Developing synthetic biological devices to allow the noninvasive control of cell fate and function in vivo can potentially revolutionize the field of regenerative medicine. To address this unmet need, we designed an artificial biological “switch” that consists of two parts: (1) electromagnetic perceptive gene (EPG) and (2) magnetic particles. Our group has recently cloned the EPG from the Kryptopterus bicirrhis (glass catfish). The EPG encodes to a putative membrane-associated protein that responds to electromagnetic fields (EMF). This gene’s primary mechanism of action is to raise the intracellular calcium levels or change in flux through EMF stimulation. Here, we developed a system for the remote regulation of [Ca2+]i (i.e. intracellular calcium ion concentration) using streptavidin-coated ferromagnetic particles (FMPs) under a magnetic field. The results demonstrate that the EPG-FMPs can be used as a molecular calcium switch to express target proteins. |
| 저자 |
황장선1, Assaf A Gilad2, 최종훈1
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| 소속 |
1중앙대, 2Michigan State Univ. |
| 키워드 |
Synthetic biological device; Electromagnetic perceptive gene; Kryptopterus bicirrhis; Calcium ion; Magnetic particles
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| E-Mail |
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