| 학회 |
한국고분자학회 |
| 학술대회 |
2004년 봄 (04/09 ~ 04/10, 고려대학교) |
| 권호 |
29권 1호, p.182 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Galactosylated Polyethylenimine -graft-poly(vinyl pyrrolidone) as a Hepatocyte-Targeting Gene Carrier |
| 초록 |
Non-viral gene carriers in gene therapy such as cationic polymers may overcome the current problems associated with viral gene carrier in safety, immunogenicity and mutagenesis. In this study, polyethylenimine (PEI) was used as a gene carrier and conjugated with lactose bearing galactose group for hepatocyte targeting and grafted with poly(vinyl pyrrolidone) (PVP) as a hydrophilic group to reduce cytotoxicity of PEI and prevent aggregation of complex from proteins in vivo. The galactosylated PEI-PVP (Gal-PEI-PVP)/DNA complex was confirmed using agarose gel electrophoresis and DNase I experiment. The sizes and zeta potentials of the complex were measured by dynamic light scattering. The cytotoxicity of Gal-PEI-PVP in HepG2 cells was evaluated. The Gal-PEI-PVP/DNA complex was transfected into HepG2 cells having asialoglycoprotein receptors (ASGRs), indication of specific interaction between ASGRs on HepG 2 cells and galactose ligands on Gal-PEI-PVP. |
| 저자 |
국승은1, 박인규1, 최윤재1, 조명행1, T.Akaike2, 조종수3
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| 소속 |
1서울대, 2Tokyo Institute of Technology, 3Japan |
| 키워드 |
gene therapy; gene delivery; PEI; PVP; galactose; nonviral vector; gene carrier
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| E-Mail |
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