| 학회 | 한국공업화학회 |
| 학술대회 | 2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 | 25권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Production of FK506 Biosynthetic Gene Clusters (BGCs) through Heterologous Strain in Streptomyces coelicolor |
| 초록 | Heterologous expression of natural product biosynthetic gene clusters (BGCs) gives an approach not only to decode biosynthetic logic behind natural product (NP) biosynthesis, but also to discover new chemicals from unknown BGCs. Transformation-associated recombination (TAR) cloning make use of the natural in vivo homologous recombination of Saccharomyces cerevisiae to directly capture large genomic loci. FK506 (tacrolimus) is known as immunosuppressant and antifungal activity, which can be produced by Streptomyces tsukubaensis. Though we have already known sequence or length of FK506 gene cluster, the synthesis of FK506 still represents several challenges. Here we report a TAR-based genetic platform that allows us to directly clone in S.cerevisiae as a cloning strain, refactor transcriptional region in biosynthetic gene clusters, and heterologous express a silent biosynthetic pathway to yield a FK506 in Streptomyces coelicolor. |
| 저자 | 구본철, 오민규 |
| 소속 | 고려대 |
| 키워드 | Streptomyces; TAR cloning; Biosynthetic gene clusters |
