| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Hyaluronic acid-ceramide (HACE)-based liposomes for enhanced gene delivery to CD44-positive cancer cells |
| 초록 |
Hyaluronic acid-ceramide (HACE)-modified liposomes were designed using DOTAP/DOPE for targeted delivery of therapeutic genes to the CD44 receptor-overexpressing cancer cells. Lipoplex was then prepared at different N/P ratios; the gel retardation test showed strong DNA binding affinity of liposomes for targeted gene delivery. Cytotoxicity of liposomes was evaluated by WST assay for different cell lines such as MDA-MB-231 and NIH3T3 cells. We then examined the transfection efficiency of liposomes using luciferase reporter plasmid DNA. We found transfection efficiency of HACE-based liposomes was remarkably higher in case of MDA-MB-231 cells as compared to NIH3T3 cells. Our findings have shown interesting prospective for tumor targeted delivery of therapeutic gene in CD44 receptor-positive cells with less cytotoxic effects. |
| 저자 |
수딥타 말릭, 최준식
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| 소속 |
충남대 |
| 키워드 |
Hyaluronic acid; CD44 receptor; Liposome; Gene therapy; Receptor-mediated endocytosis
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| E-Mail |
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