| 초록 |
Peptides have been used in various biomedical fields including gene and drug delivery systems. In this study, multifunctional peptide dimer (MPD) was utilized for gene delivery systems. The peptide monomer consists of cellular penetrating peptide moiety (R8), MMP-2 specific sequence (GPLGV), pH-responsive moiety (H5) and hydrophobic moiety (palmitic acid) (CR8GPLGVH5-Pal). MPD was formed by DMSO oxidation of thiols in terminal cysteine residues. MPD could condense pDNA successfully at a weight ratio of 2. MPD itself could self-assemble into sub-micron particles via hydrophobic interaction. MPD showed low cytotoxicity in comparison with PEI25k. MPD polyplex showed low transfection efficiency in HEK293 cells expressing no MMP-2 but high transfection efficiency in A549 cells expressing MMP-2, meaning the enhanced transfection efficiency by MMP-induced structural change. This result shows the potential of MPD for targeted gene delivery systems. |
