| 초록 |
We investigated the expression pattern of genes in zebrafish embryos after exposure to silver (Ag), copper-doped titanium dioxide (Cu-TiO2), and titanium dioxide (TiO2) Nanoparticles (NPs) and Nanotubes (NTs). A total of 314 (278 up-regulated and 36 down-regulated), 283 (129 up-regulated and 154 down-regulated), and 360 (198 up-regulated and 162 down-regulated) genes were differentially expressed in zebrafish embryos exposed to Ag, Cu-TiO2, and TiO2 NPs, respectively, with apoptosis being the function of the highest proportion of differentially expressed genes in all 3 NP exposures. TiO2 NPs and NTs induced asymmetric regeneration of fins at 1 mg/L, and apoptosis was observed in zebrafish embryos exposed to TiO2 NPs and NTs at 20 ng/L. Significantly more TiO2-NPs and TiO2-NTs accumulated in reproductive organs and intestines of fish with amputated fins, suggesting that TiO2-NPs and TiO2-NTs cause undesired tissue accumulation and toxicity. |
