초록 |
This study using a protease was measured according to the molecular weight of the antihypertensive activity cucumber protein hydrolysate. The enzyme Transglutaminase, Protamax, Neutrase, Pepsin, Alcalase using a total of five enzyme. A single enzyme and mix enzyme divided into 12 different groups (TGA, TGAN, A, AN, TGP, TGPN, P, PN, TGPe, TGPeN, Pe, PeN) were hydrolyzed. Using ultrafiltered samples of 30KDa, 10KDa, 30~10KDa. The hydrolyzate was used for the measurement and the supernatant freeze-dried and centrifuged. Using HPLC and spectrophotometer by measuring the ACE inhibitor effect To evaluate the antihypertensive activity of the protein hydrolyzate of the molecular weight of the sea cucumber. |