| 초록 |
Microbial fermentation is a promising method that can overcome major bottlenecks in conventional methods of lycopene production, such as low yields and poor quality. To further improve lycopene production, it is important to utilize metabolic enzymes with high specific activities. Two enzymes, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and farnesyl diphosphate synthase (IspA), are essential in lycopene production using MEP pathway. Here, we demonstrated the superior activities of Dxs and IspA of Vibrio sp. dhg, a newly isolated and fast-growing microorganism. We found that Dxs and IspA in Vibrio sp. dhg showed 1.08-fold and 1.38-fold higher catalytic efficiencies, respectively. Consequently, the heterologous overexpression increased the specific lycopene production by 1.88-fold. Our findings could be widely used to increase production of lycopene and other carotenoids. |
