| 초록 |
Microbial fermentation is a promising method that can solve major problems in original methods of lycopene production, such as low yields and poor quality. To enhance lycopene production, it is important to utilize metabolic enzymes with high reactivity. Two enzymes, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and farnesyl diphosphate synthase (IspA), are crucial in lycopene production using MEP pathway. Here, we demonstrated the superior activities of Dxs and IspA of Vibrio sp. dhg, a newly discovered microorganism. We found that Dxs and IspA in Vibrio sp. dhg showed 1.08-fold and 1.38-fold higher catalytic efficiencies, respectively. Consequently, the heterologous overexpression enhanced the specific lycopene production by 1.88-fold. Our findings could be widely used for production of lycopene and other carotenoids in microbes. |
