| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Enhanced lycopene production in Escherichia coli by expression of two MEP pathway enzymes from Vibrio sp. dhg |
| 초록 |
Metabolic engineering is apromising method that can overcome major problems in conventional methods oflycopene production, such as low yields and poor quality. To enhance lycopeneproduction, it is important to introduce metabolic enzymes with high specificactivities. Two MEP pathway enzymes, 1-deoxy-d-xylulose-5-phosphate synthase(Dxs) and farnesyl diphosphate synthase (IspA), are essential in lycopeneproduction. Here, we verified the high enzyme activities of Dxs and IspA of Vibriosp. dhg, a newly isolated and fast-growing microorganism. We found that Dxs andIspA in Vibrio sp. dhg showed 1.08-fold and 1.38-fold higher catalyticefficiencies, respectively. Consequently, the overexpression of two MEP pathwayenzymes increased the specific lycopene production by 1.88-fold. Ourfindings could be widely used to increase production of lycopene and othercarotenoids. |
| 저자 |
이혜경, 정규열, 김민재, 노명현, 우성화, 임현규
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| 소속 |
포항공과대 |
| 키워드 |
lycopene; Vibrio sp.dhg; MEP pathway
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| E-Mail |
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