| 초록 |
In this paper, three aminobenzoic acids with different directivity such as ortho-, para-, meta- were separated using different types of ionic liquids as an additive in the mobile phase in HPLC. And the three ionic liquids, 1-butyl-3-methylimidazolium tetrafuloroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafuloroborate ([EMIm][BF4]), and 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) were used. From these ionic liquids, [BMIm][BF4] seemed to be the most suitable, because of the good resolution. Between the samples, the mobile phase composition was 25 vol. % of modifier (water:methanol=75:25 (vol. %)) with different concentrations of each ionic liquid. Separation of aminobenzoic acids was done on commercially available octadecyl silica column (4.6×150 mm i.d. and particle size 5 µm). Effects of counterions on different ionic liquids and their concentrations in the retention and separation of aminobenzoic acids were discussed and interpreted on the basis of intermolecular interactions. |
