| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.279 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Surface Characteristics and Cell Adhesion Behavior of Acetylated Chitosan Films : Effect of Degree of Acetylation |
| 초록 | Chitosan is a non-toxic and biocompatible cationic polymer. Chitosan is a linear heteropolysaccharide composed of β-1,4-linked D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) with various compositions of these two monomers.1 The degree of acetylation (DA) represents the portion of GlcNAc units with respect to total number of units. The DA of chitosan influences not only the physicochemical characteristics, but also the biological properties of chitosan.2 In this study, we synthesized DA-controlled chitosans (from 10% to 90%) by the acetylation reaction of deacetylated chitosan (DA ∼ 10%) and acetic anhydride with different ratio. Their surface properties were investigated by the measurements of water contact angle and zeta potential as well as the chemical structures by FTIR-ATR. The cell compatibility of the acetylated chitosans with different DA value was estimated by in vitro cell culture using NIH/3T3 fibroblasts and C28/I2 human chondrocytes (cell seeding density, 4 × 104/cm2). It was observed that the cell adhesion and growth decreased on the acetylated chitosan film surfaces with increasing DA value, probably owing to the increased number of acetyl groups leading to the increased hydrophobicity (그림 1, 2). Acknowledgment This work was supported by a grant from Korea Ministry of Commerce, Industry and Energy. References 1. A. Domard and M. Rinaudo, Int. J. Biol. Macromol., 5, 49 (1983). 2. C. Chatlet, O. Damour, and A. Domard, Biomaterials, 22, 261 (2001). |
| 저자 | 송대근1, 오세행1, 이동신2, 배은희2, 이진호1 |
| 소속 | 1한남대, 2(주) 리젠바이오텍 |
| 키워드 | Acetylated chitosan; Degree of acetylation; Surface property; Cell compatibility |
