| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.412 |
| 발표분야 |
생물화공 |
| 제목 |
Preparation of Fe3O4@metal-silicate core-shell nanospheres for His-tagged protein separation |
| 초록 |
Separation of His-tagged protein taking advantage of immobilized-metal affinity chromatography (IMAC) or using magnetic nanoparticles attracted enormous interests in the exact purification of the recombinant protein, but the selection of optimized metal ion has been reported infrequently. Including histidine, each amino acid exhibits different affinity or specificity with diverse metal ion so that the selection of suitable metal ion can be an important issue. Herein, we successfully synthesized Fe3O4@metal-silicate core-shell nanospheres doped with diverse metal ion, Mn2+, Co2+, Ni2+ and Cu2+. Sea urchin shaped (spiky) sparse metal-silicate covered super-paramagnetic nanospheres provided effective protein binding sites to which enabled efficient metal affinity attachment of His-tagged protein resulting in rapid separation as well as convenient application of external magnetic field. In addition, detachment of His-tagged protein was available using excess imidazole solution and Fe3O4@metal-silicate nanospheres could be reusable. Finally, we reported comparison study of His-tagged protein purification efficacy according to various metal ion types. |
| 저자 |
신무광1, 강병훈1, 김명훈1, 한승민1, 윤남경2, 안정오2, 함승주1
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| 소속 |
1연세대, 2한국생명공학(연) |
| 키워드 |
Histidine; metal silicate; magnetic separation; protein purification; affinity
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| E-Mail |
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| 원문파일 |
초록 보기 |
