| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Fabrication of multi-functional hydrogel based 3D co-culture system for enhancing osteogenesis by paracrine effect |
| 초록 |
In this study, paracrine effect between human adipose-derived mesenchymal stem cells (hADSCs) and osteoblasts was systemically investigated in the collagen-based three-dimensional scaffolds for bone regeneration. Osteoblasts-encapsulated microbeads using electrostatic droplet extrusion were embedded with hADSCs in collagen bulk hydrogel scaffolds for indirect co-culture system. We hypothesized that factors secreted from osteoblasts have an influence on osteogenic differentiation of hADSCs by the paracrine effect. We prepared bare and bone morphogenetic protein 2 (BMP-2) containing alginate beads for control groups. This co-culture system in compartmentalized hydrogel based scaffold, osteogenic marker such as ALP, ARS, OCN were increased compared to hADSCs monoculture system. This hydrogel based culture system can potentially be used to study the paracrine effect for various tissue regeneration. |
| 저자 |
김혜림1, 국윤민2, 이경미1, 박민희1, 최영빈1, 이재협1, 고원건2, 이강원1
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| 소속 |
1서울대, 2연세대 |
| 키워드 |
stem cell; osteogenesis; 3D scaffold; hydrogel; bone regeneration; paracrine effect
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| E-Mail |
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