| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.264 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Evalution of biocompatibility ; Crosslinked alginate - SIS Sponge |
| 초록 | SIS is the natural biomaterials mainly composed of collagen, glycosaminoglycan and growth factors. Tissue engineering techniques have been investigated the possibility of the application of small intestine submucosa (SIS) for a wound dressing. It has been recently recognized that SIS has been examined as organ reconstructions without immuno-rejection response1. This study described the preparation and characterization of a three-dimensional and porous sponge made from alginate2 and SIS3. SIS solutions of 1 and 2 wt% with 0.1% pepsin in 3% acetic acid were reacted for 48 hrs and freeze-dried. They were treated with different concentrations (50 mM and 100 mM) of 1-ethylene-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) for 24 hrs and lyophilzed. Then 1 wt% alginate solution was pass through the SIS sponge and lyophilzed. Calcium chloride (CaCl2) was used as crosslinking agent. The prepared sponges were characterized by scanning electron microscopy (SEM), differential scanning calorimeter (DSC) analysis, Fourier transform infrared spectrometer (FT-IR), porosity, and water uptake ability. The biocompatibility of NIH/3T3 with these SIS sponges was found to be acceptable at a cell density of 2×104 cell/cm2. We investigated biodegradation of SIS sponge, MTT assay and H ＆ E stain. From the MTT assay results, the biocompatibility of the crosslinked SIS sponge with NIH/3T3 was confirmed. Acknowledgement : This work was supported by Korea Ministry of Commerce, Industry and Energy (N11-A08-1402-05-1-3). Fig. 1. SIS sponge section (×300). Fig. 2. Alginate-SIS sponge section (×300). Reference 1. K. Lindberg and S. F. Badylak, Burns, 27, 254 (2000). 2. L. Shapiro and S. Cohen, Biomaterials, 18, 583 (1997). 3. H. W. Shin, S. H. Kim, J. W. Jang, M. S. Kim, S. H. Cho, H. B. Lee, and G. S. Khang, Polymer Korea, 28, 194 (2004). |
| 저자 | 홍금덕1, 김순희2, 김선경2, 고연경2, 강길선2, 김문석1, 조선행1, 이해방1 |
| 소속 | 1한국화학(연), 2전북대 |
| 키워드 | biocompatibility; alginate-SIS sponge |
