| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 봄 (04/10 ~ 04/12, 부산컨벤션센터(BEXCO)) |
| 권호 |
44권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Design of Three Color Polymer Dots via Fluorescence Resonance Energy Transfer for Multi-Color Imaging |
| 초록 |
Polymer dots (Pdots) represent fluorescent probes for bioimaging because of high brightness, low cytotoxicity, good photostability. To produce different emission wavelength of Pdots (green, red and near-infrared), we utilized green-emissive conjugated polymer alone or blend with red or NIR fluorophores to adopt fluorescence resonance energy transfer (FRET). During the formation of Pdots, polymer matrix (polystyrene maleic anhydride) was co-precipitated with fluorescent semiconducting materials, enabling efficient FRET due to 1) close distance between FRET donor and acceptor as well as 2) suppressed aggregation-caused quenching, prohibiting the serious packing of fluorophores. Subsequently, the amide bond formation between the carboxyl groups on the Pdot surface and the amine groups of target-specific antibodies was catalyzed by COMU. Upon excitation with only one wavelength, three color polymer dots can distinguish different organisms in the cell simultaneously. |
| 저자 |
염경빈, 정지은, Uddin Mohammad Afsar, 유화숙, 심상희, 우한영
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| 소속 |
고려대 |
| 키워드 |
conjugated molecules; polymer dot; FRET; targeting; imaging
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| E-Mail |
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