| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1470 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Production of 3-aminopropionic acid by metabolically engineered Escherichia coli |
| 초록 |
3-aminopropionic acid (3-AP) is important platform chemical for the production of acrylamide and acrylonitrile. The previously developed fumaric acid producing E. coli strain was used as base strain. To convert L-aspartate to 3-AP, the C. glutamicum panD gene (encoding L-aspartate-α-decarboxylase) was overexpressed. The native promoter of the aspA gene was replaced with the trc promoter to increase flux to aspartate. Also, the ppc gene (encoding phosphoenolpyruvate carboxylase) was overexpressed and ammonium sulfate was supplemented in the medium. Then, 3.49 g/L 3-AP was produced. The 3-AP titer was increased to 3.94 g/L through optimization of PPC expression level by using synthetic promoters and RBS sequences. To reduce acetic acid accumulation, native promoter of the acs gene was replaced with the trc promoter. Fed-batch cultures of final strains resulted in the production of 32.3 g/L 3-AP in 39 h. (This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557)). |
| 저자 |
이언희1, 송찬우2, 이종민3, 고유성1, 이상엽1
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| 소속 |
1KAIST, 2GS Caltex, 3한국해양과학기술원 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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