| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.2476 |
| 발표분야 |
4차 산업혁명에서 바이오의약품 연구 개발 동향 심포지엄(생물화공부문위원회) |
| 제목 |
Deep Sequencing of Antibody Repertoire for Biomedical Application |
| 초록 |
The advent of next-generation sequencing (NGS) technologies has allowed us to determine antibody repertoires at an unprecedented depth. In this talk, I will present two approaches that employed NGS for investigating antibody repertoire. First, I will discuss antibody sequencing to determine the naïve and antigen-experienced repertoires of healthy human donors by using a recently developed large-scale experimental method for pairing heavy and light chain variable regions. Second, I will introduce the high-throughput sequencing coupled with mass spectrometry of serological antibodies. This proteogenomic approach allowed us to quantitatively characterize the influenza-specific serological antibody repertoire. These two approaches for antibody repertoire will provide an advance in studying antibody responses to disease or to vaccination, aiding the development of therapeutic antibodies. |
| 저자 |
박대찬 |
| 소속 |
한국과학기술(연) |
| 키워드 |
next-generation sequencing; antibody repertoire
|
| E-Mail |
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| 원문파일 |
초록 보기 |
