| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Improved Pterostilbene Production by Co-culture of E.coli and Streptomyces venezuelae |
| 초록 |
Pterostilbene is an effective anti-tumor and antioxidant agent as a resveratrol derivative with two additional methyl branch, thus has significant advantages over resveratrol. The biosynthesis of pterostilbene is derived from phenylpropanoid pathway. The p-coumaric acid is converted to p-coumaric CoA by p-coumarate CoA ligase (4CL) and it combines with three malonyl-CoA to produce resveratrol by stilbene synthase (STS). Next, by series action of 3,5-O-methyltransferase (ROMT), pterostilbene is produced. In this study, 4CL and STS coding genes were transformed into E.coli BL21 (DE3) and ROMT coding gene into Streptomyces venezuelae DHS2001, respectively. Finally, the pterostilbene production was enhanced when the recombinants were incubated in the co-culture system. This result provides the potential utility of the co-culture system for efficient pterostilbene manufacturing. |
| 저자 |
이나령, CUI HEQING, 송명종, 신혜령, 이지영, 조진철, 윤여준
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| 소속 |
이화여자대 |
| 키워드 |
Pterostilbene; Co-culture; Production
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| E-Mail |
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