| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo |
| 초록 |
Currently, because of their highly evolved and specialized components, viral systems are by far the most effective means of gene delivery and expression. Covalent modification of proteins and enzymes with activated poly(ethylene glycol) (PEG) is expected to be a good candidate for the hydrophilic part due to the high solubility in water, low cytotoxicity, and enhanced cell permeability. Recombinant baculovirus vectors engineered to contain mammalian cell-active promoter elements have been applied to the delivery of genes into mammalian cells such as hepatocytes and neurons. In this study, ApEGFP-N2 and ApSVLuc vectors were constructed and plaque-purified. Transduction efficiency with pegylated baculovirus containing GFP or luciferase gene was determined on several cell lines. Also, ICR mice were inoculated with pegylated baculovirus containing GFP gene. In conclusion, transduction efficiency was regulated by pegylation of baculovirus vector in vitro and in vivo. |
| 저자 |
김유경, 박인규, 강호림, 최재영, 제연호, 김화, 김현우, 조명행, 조종수
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| 소속 |
서울대 |
| 키워드 |
baculovirus; PEGylation; viral vector; gene carrier
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| E-Mail |
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