| 초록 |
Adenosine triphosphate (ATP) is a bioenergy for operating metabolisms and maintaining cell survival and helps drug delivery systems to escape from the endo/lysosomes. This study synthesized three ATP polymers with degradable bonds triggered by either enzyme or enzyme/thiol. The endosome-escaping and bioenergetic effects of different bioreduction rates in biodegradable non-reducible ATP polymer (pATP), partially-reducible polyATP (PRpATP), and fully-reducible polyATP (FRpATP)) were evaluated by gene transfection efficiency and cell survival rate, respectively of ATP polymer-loaded polyplexes. Incorporation of PRpATP or FRpATP in bPEI25kDa-based gene complexes exhibited higher gene transfection efficiency than no addition. Especially, in energy-deficient conditions, PRpATP or FRpATP-incorporated polyplexes showed high transfection efficiency and improved cell viability. Thus, biodegradable ATP polymers could have potentials in polymeric gene therapy and energy-deficient applications. |
