| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.352 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Tissue Engineered Biodisk using Natural/Synthetic Mesohybrid Scaffolds |
| 초록 | Study on tissue engineered biodisk as the substitute therapy for the degeneration of the intervertebral disc composed of two distinct regions, the inner soft and highly hydrated nucleus pulposus and the outer firm annulus fibrosus is active. Actually the regeneration of nucleus pulposus on the upper surface of a porous bone substitute material (Calcium polyphosphate (CPP)) was performed by Rita A. Kandel et. al. We tried to prepare Biodisk based on mesohybrid scaffolds included a new bone formation material, DBP. Demineralized bone particle (DBP) impregnated with bone morphogenetic proteins (BMP) takes effect as the powerful inducer of a new bone growth. It has been reported that the above-stated BMP expedites the differentiation of bone marrow stromal cells (BMSCs) into bone and cartilage. Poly(lactide-co-glycolide) (PLGA), one of biodegradable polymers, has been used widely for tissue engineering, recently. Mesohybrid scaffolds created with natural/synthetic materials by solvent casting/salt leaching method is 5mm diameter and 5mm height. In order to analysis the surface and the inner structure scaffolds were observed by means of SEM and the average pore size of them was 250㎛. In this study cells collected directly from disks of distal caudal spines were used. Cells were seeded on scaffolds in the specified medium according to the known procedure and then harvested at 2, 4, 6 weeks to analyze the histologic appearance, the water content, proteogylcan and collagen contents, etc. (a) (b) Figure 1. SEM photographs of the surface (a) and the inner structure (b) of mesohybrid scaffolds with DBP 40%. 참고 문헌 1. M. R. Urist, Science, 150, 893 (1965) 2.G. Khang, C. S. Park, J. M. Rhee, S. J. Lee, Y. M. Lee, I. Lee, M. K. Choi, and H. B. Lee, Korea Polym. J., 9, 267 (2001) Acknowledgement : This work was supported by KMOHW (0405-BO 01-0204-0006). |
| 저자 | 문대식1, 김순희1, 김은정2, 조윤경3, 양대혁1, 고연경1, 고종태1, 김연식1, 나현석1, 이승현1, 김문석2, 한창환4, 이종문1, 강길선1 |
| 소속 | 1전북대, 2한국화학(연), 3카톨릭의과대, 4카톨릭 의대 정형외과 |
| 키워드 | Tissue engineered biodisk; Nucleus pulposus; Calcium polyphosphate; Natural/synthetic mesohybrid scaffolds; DBP; PLGA |
