| 초록 |
Despite of the great attention for developing cancer therapy, especially targeting Cancer Stem Cell (CSC), CSC-related research have been gone through struggles by the limited supply of diverse, patient-derived CSC. Here, we developed polymer thin film (PTF) platform that promotes conversion of cancer cells to cancer stem cells-enriched tumor spheroid which shows highly tumorigenic property without biochemical or genetic manipulations. PTF-induced cancer spheroids showed a significant increase in the expression of CSC-associated genes and exhibited robust drug resistance compared with monolayercultured controls. These spheroids also exhibited highly tumorigenic property in vivo, especially enhanced xenograft tumor-forming ability and metastatic capacity. Our results suggest that by providing a new method of inducing CSC-like tumor spheroids from diverse cancer cells, the PTF platform described here will contribute to CSC-related reseach and drug development. |
