| 학회 | 한국공업화학회 |
| 학술대회 | 2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 | 24권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Artificial lactam riboswitch as an intracellular metabolite sensor for high-throughput screening and selection |
| 초록 | Caprolactam is is a cyclic amide of caproic acid and is widely used to produce nylon-6 through ring-opening polymerization. An intracellular biosensor for caprolactam can facilitate high-throughput metabolic engineering of recombinant microbial strains. Because of the mixed production of caprolactam and valerolactam in the recombinant strain, a caprolactam biosensor should be highly specific for caprolactam. However, a highly specific caprolactam sensor has not been reported. Here, we developed a specific caprolactam riboswitch useful for monitoring the intracellular concentration of caprolactam. The riboswitch developed through coupled in vitro−in vivo selection responded specifically to caprolactam. Finally, caprolactam-dependent control of cell growth was demonstrated using the riboswitch. This riboswitch is a valuable tool for directed evolution of enzymes and pathway optimization for the microbial production of caprolactam. |
| 저자 | 정금영, 장성연, 장성호, 정규열 |
| 소속 | 포항공과대 |
| 키워드 | caprolactam; riboswitch; metabolite biosensor; high-throughput screening |
