| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of caprolactam-specific RNA based biosensor |
| 초록 |
Caprolactam is a monomer of nylon-6, a high-tensile material of synthetic fiber. An intracellular biosensor for caprolactam can facilitate high-throughput metabolic evolution of microbial strains. Because of the mixed production of caprolactam and valerolactam in the recombinant strain, a biosensor should specifically recognize the structure of caprolactam. However, a highly specific caprolactam biosensor has yet been reported. Here, we developed an artificial riboswitch that meets the need for sensor-guided evolution. Coupled in vitro-in vivo library selection strategy was applied for the development of the translation-initiation regulating sensor. Selected caprolactam riboswitch successfully discriminated caprolactam from valerolactam and enabled caprolactam-dependent control of cell growth. Further applications in microbial cell factory for caprolactam production using caprolactam biosensor is expected. |
| 저자 |
김민선1, 장성연2, 장성호3, 임대균4, 강택진5, 오민규4, 정규열1
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| 소속 |
1포항공과대, 2Weill Cornell Medicine, 3인천대, 4고려대, 5동국대 |
| 키워드 |
caprolactam; aptamer; SELEX; riboswitch
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| E-Mail |
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