| 초록 |
The surface of glassy carbon electrode (GCE) was initially modified with amino groups using electrooxidation of ammonium carbamate at a potential exceeding 1.0 V vs Ag/AgCl. Interestingly, aminated GCE exhibits capability of electrooxidizing carbamic acid produced from urea during urease reaction. It is, however, always a great practical challenge of how to efficiently and stably immobilize urease on aminated GCE to improve electrode stability and lifetime. Herein, urease was immobilized onto aminated GCE using glutaraldehyde and aniline as cross-linking and co-polymerization agents, respectively. The prepared urease-based aminated GCE was employed for urea sensing using amperometric and cyclic voltammetric (CV) techniques. A high sensitivity and good linear relationship between urea concentration and current were observed on the prepared bioelectrode. |
