| 초록 |
We studied that myocardial protection of infracted heart using dual growth factor delivery with self-assembling peptide nanofiber hydrogel (SAP). The SAP, RADAII-16, forms fibers (5 to 10 nm) and assembles into a 3D scaffold at physiological pH. Myocardial infarction (MI) was produced in ~ 150g SD rat. After left coronary artery ligation, growth factor with SAP was injected into infracted border zone (n=6 in each group: Sham, MI, MI + SAP, MI + SAP/FGF-2, MI + SAP/PDGF-BB, MI + SAP/FGF-2/PDGF-BB, and FGF-2/PDGF-BB). Injection of SAP could prevent cardiac fibosis and ~45 % of cardiomyocyte apoptosis were reduced 4 and 8 weeks after surgery. The infarct size of dual growth factor with SAP was dramatically reduced and arterial vessel area, especially, was statistically no difference with sham group. Moreover, cardiac function of this group was almost recovered. In conclusion, dual growth factor with SAP is effective to myocardial protection and leads to improvement in cardiac function. |
