| 초록 |
Hyaluronic acid is gaining popularity as a biomaterial scaffold in tissue engineering field due to its biocompatibility and its presence in the extracellular matrix of tissues. However, it needs a specific treatment to crosslink the material in order to improve its mechanical properties. Below 40℃, Agar forms a non-cytotoxic hydrogels and is often used as a support for the tri-dimensional culture of human cells. For this reason it can be utilized to reproduce the extracellular matrix (ECM) in the hydrogel and to support the proliferation and differentiation of chondrocytes for cartilage regeneration. In this study, agar (2wt%) solutions with the addition of 0, 0.25, 0.5, 0.75, 1 wt% of hyaluronic acid were prepared to study the effect of hyaluronic acid on chondrocytes. The scaffolds were characterizations by FT-IR, compressive strength, SEM for porosity, then the cell proliferation was evaluated by MTT and Confocal. |
