| 초록 |
In bioprinting technology, gelatin, which has high biocompatibility and biodegradability properties, is a good candidate for bioinks. However, gelatin has low printability due to its low viscosity, and the chemical cross-linking used to maintain the 3D shape can cause cytotoxicity. In this study, we optimized the rheological properties of gelatin and cross-linking methods to improve printability and cell affinity. We fabricated the 3D scaffolds with high strand uniformity at the optimum gelatin concentration and melt temperature. Then the 3D scaffolds were cross-linked using the physical and chemical method to maintain the 3D structure. We have confirmed the morphology of the 3D scaffolds using SEM. In order to evaluate the cell affinity, HDFs were cultured on the 3D scaffolds. In DNA assay, we confirmed that the physical cross-linked 3D scaffolds have approximately 44% higher cell proliferation rate than that of the 3D scaffolds using the chemical cross-linking. |
