| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
10권 2호, p.1599 |
| 발표분야 |
생물화공 |
| 제목 |
Development of serum-free medium for recombinant CHO cells producing recombinant antibody |
| 초록 |
One of the most important considerations in animal cell cultivation is in determining the composition of the cell culture medium. Also, there has been extensive efforts to identify suitable alternatives to serum components. It is desirable to use serum-free medium due to the cost-effective supply of media and consistent production of therapeutic protein. Serum-free medium can be optimized for the physiological conditions in order to maximize cell growth and productivity of therapeutic protein. Among mammalian cells, chinese hamster ovary (CHO) cells are one of the most frequently used cell lines for the expression of recombinant proteins that require post-translational modification to express full biological function. CHO cell line which are dihydrofolate reductase deficient (dhfr-) have been used extensively for this purpose. In this study, a serum-free medium were developed for the production of recombinant antibody by rCHO cells and various components for the fortified medium were evaluated to enhanced the cell growth and productivity of recombinant antibody. |
| 저자 |
김도윤1, 이준철1, 장호남2, 오덕재3
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| 소속 |
1한국과학기술원 / 세종대, 2한국과학기술원, 3세종대 |
| 키워드 |
CHO; recombinant antibody; media
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| E-Mail |
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| 원문파일 |
초록 보기 |
