| 초록 |
Specific cell-targeting ligands can be conjugated to polymeric gene carriers to enhance target specific gene delivery. Artery wall binding peptide (AWBP), a specific cell targeting peptide, was conjugated to poly(ethylene glycol)-grafted-poly(L-lysine)(PEG-g-PLL) to enhance the gene transfer to artery wall cells. 1H-NMR analysis was confirmed the composition of the obtained polymer and indicated that four moles of AWBP were reacted to one mole of VS-PEG-PLL. The particle size and the shape of AWBP-PEG-PLL/pDNA complexes were measured by DLS and AFM, respectively. The particles of AWBP-PEG-PLL/pDNA complexes were spherical with a size of ～100 nm. It was found in agarose gel retardation assay that AWBP-PEG-PLL was able to condense plasmid DNA and reach complete complexation at and above of a charge ratio 1/1(+/-). Transfection efficiency of AWBP-PEG-PLL/pDNA complexes was 150～180 times higher than that of control systems. Based on this study, it was concluded that a novel gene delivery carrier was successfully synthesized and displayed an important role in the efficient gene delivery to the arterial wall cells. |
