| 초록 |
Chemotaxis is movement of an organism in response to a chemical signal. In this study, we observed the chemotactic behavior of a motile microalga, Chlamydomonas reinhardtii, in various systems. According to the previous studies, Chlamydomonas reinhardtii utilizes carbon dioxide (CO2) and bicarbonate ion (HCO3-) as carbon sources at the same time. It is expected that the chemotactic response to HCO3- is related to the CO2 fixation efficiency. Firstly, we devised a flow-free microfluidic system and observed the chemotactic response to the different concentrations of HCO3-. As a result, we found the most preferred concentration is 30mM. Based on this result, we designed a flowing system for strain screening. Well-growing mutants showed a tendency to prefer higher concentration. This result implies that the strategy can be used for high-throughput screening to find the superior microalgal strains with high efficiency of CO2 fixation. |
