학회 | 한국고분자학회 |
학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
권호 | 29권 2호, p.98 |
발표분야 | 고분자 구조 및 물성 |
제목 | The Osmotic Pressure of Aqueous Globular Protein Solutions : Ovalbumin/salt and Bovine serum albumin(BSA)/salt solutions |
초록 | Osmotic pressure , important property in the interpretation of biophysical systems , is determined from various conditions. Especially in an aqueous protein solutions , the osmotic pressure can be used to characterize interactions between globular protein and salt molecules. In the meanwhile , the method for experimentally determining cloud-point curves of liquid protein solution is provided by thermooptical analysis(TOA). The cloud-point temperature(CPT) is measured as functions of salt’s type and concentration at constant pH for ovalbumin/salt and bovine serum albumin(BSA)/salt solutions. The interaction energy between various protein’s surface of amino acid sequence and salt’s ion is contributed to the osmotic pressure. The energy parameter expressed as a function of ionic strength is obtained from CPT data in the equation of state based on liquid-liquid equilibrium conditions and used to describe the osmotic pressure through relatively simple thermodynamic model. |
저자 | 최상하1, 배영찬2 |
소속 | 1한양대, 2서울시 성동구 행당 1동 한양대 |
키워드 | ovalbumin; BSA; osmotic pressure |