화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2004년 가을 (10/08 ~ 10/09, 경북대학교)
권호 29권 2호, p.98
발표분야 고분자 구조 및 물성
제목 The Osmotic Pressure of Aqueous Globular Protein Solutions : Ovalbumin/salt and Bovine serum albumin(BSA)/salt solutions
초록 Osmotic pressure , important property in the interpretation of biophysical systems , is determined from various conditions. Especially in an aqueous protein solutions , the osmotic pressure can be used to characterize interactions between globular protein and salt molecules.
In the meanwhile , the method for experimentally determining cloud-point curves of liquid protein solution is provided by thermooptical analysis(TOA). The cloud-point temperature(CPT) is measured as functions of salt’s type and concentration at constant pH for ovalbumin/salt and bovine serum albumin(BSA)/salt solutions.
The interaction energy between various protein’s surface of amino acid sequence and salt’s ion is contributed to the osmotic pressure.
The energy parameter expressed as a function of ionic strength is obtained from CPT data in the equation of state based on liquid-liquid equilibrium conditions and used to describe the osmotic pressure through relatively simple thermodynamic model.
저자 최상하1, 배영찬2
소속 1한양대, 2서울시 성동구 행당 1동 한양대
키워드 ovalbumin; BSA; osmotic pressure
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