학회 |
한국고분자학회 |
학술대회 |
2015년 가을 (10/06 ~ 10/08, 대구컨벤션센터(EXCO)) |
권호 |
40권 2호 |
발표분야 |
X-Ray & Neutron Scattering for Polymer Science |
제목 |
Conformational Transition of Membrane-Associated Terminally Acylated HIV-1 Nef |
초록 |
Many proteins are posttranslationally modified by acylation targeting them to lipid membranes. While methods such as X-ray crystallography and nuclear magnetic resonance are available to determine the structure of folded proteins in solution, the precise position of folded domains relative to a membrane remains largely unknown. We used neutron and X-ray reflection methods to measure the displacement of the core domain of HIV Nef from lipid membranes upon insertion of the N-terminal myristate group. Nef is one of several HIV-1 accessory proteins and an essential factor in AIDS progression. Upon insertion of the myristate and residues from the N-terminal arm, Nef transitions from a closed-to-open conformation that positions the core domain 70 Å from the lipid headgroups. This work rules out previous speculation that the Nef core remains closely associated with the membrane to optimize interactions with the cytoplasmic domain of MHC-1 |
저자 |
Sushil Satija |
소속 |
NIST Center for Neutron Research |
키워드 |
HIV; Neutron Reflectivity; Protein; Membrane
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E-Mail |
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