| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
고분자 구조 및 물성 |
| 제목 |
Effect of C60 Fullerene on the i-motif DNA with complementary DNA in solution using Synchrotron X-ray scattering |
| 초록 |
The structural effects of fullerene on i-motif DNA were investigated by characterizing the structures of fullerene-free and fullerene-bound i-motif DNA, in the presence of cDNA in solutions of varying pH, using circular dichroism and synchrotron small-angle X-ray scattering. To facilitate a direct structural comparision between the i-motif and duplex structures in response to pH stimulus, we developed atomic scale structural models for the duplex and i-motif DNA structures, and for the C60/i-motif DNA hybrid associated with the cDNA strand, assuming that the DNA strands are present in an ideal right-handed helical conformation. We found that fullerene shifted the pH-induced conformational transition between the i-motif and the duplex structure, possibly due to the hydrophobic interactions between the terminal fullerenes and an internal TAA loops in the DNA strand. The hybrid structure showed a dramatic reduction in cyclic hysteresis. |
| 저자 |
김미희, 노예철, 안병철, 정성민, 박삼대, 김진철, 김동민, 정정운, 권원상, 김경태, 고용기, 최준만, 이문호
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| 소속 |
포항공과대 |
| 키워드 |
i-motif DNA; fullerene; small-angle X-ray scattering
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| E-Mail |
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