| 초록 |
Lipid-based nanoparticles can be used as versatile, biocompatible delivery vehicles for lipophilic anti-cancer drugs. Here we suggest a core-shell lipid nanocarrier encapsulating lipiodol for simultaneous delivery of drug and gene, and imaging contrasting agents. New cationic lipid nanoparticles as a drug/gene carrier were reconstituted using linear PEI conjugated with cholesterol, showing excellent dispersion stability in serum. These lipid nanoparticles contained paclitaxel in the core region, and Bcl-2 siRNA on the cationic shell. Confocal microscopy and flow cytometry analyses showed our lipid-based nanoparticles can be used as an effective vehicle for co-delivery of drugs and siRNA. Effective cytotoxic activity and apoptosis induction were also demonstrated using MCF-7 cells. We expect these lipid-based nanoparticles provide an effective means for combinational therapies, as they exhibit excellent structural stability, allowing long-term circulation and delivery to a tumor site. |
