| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
20권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Direct conversion of fibroblasts into neurons by mRNA delivery |
| 초록 |
The direct conversion of somatic cells into induced neurons (iNs) has great therapeutic potential for neurodegenerative diseases. Common direct conversion strategies to deliver several factors into somatic cells use viruses. However, novel gene delivery systems should be developed to generate iNs for safe human clinical applications. We investigated whether graphene oxide-polyethylenimine (GO-PEI) complexes are an efficient and safe system for mRNA delivery for direct conversion into iNs. The GO-PEI complexes exhibited a low cytotoxicity, high delivery-efficiency, and non-integration features for direct conversion into iNs. Moreover, in vivo transduction of reprogramming factors with GO-PEI complexes into the brain was sufficient to facilitate conversion into iNs, which alleviated symptoms in mouse models of Parkinson’s disease. Thus, the GO-PEI delivery system could be used to obtain safe iNs, and are promising for direct reprogramming-based therapies of neurodegenerative diseases. |
| 저자 |
백순봉1, 오재서2, 추연웅2, 김병수2, 김종필1
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| 소속 |
1동국대, 2서울대 |
| 키워드 |
Graphene oxide-polyethylenimine (GO-PEI); Direct conversion; Fibroblasts; Neuron; Gene delivery; Non-integration; mRNA
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| E-Mail |
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