| 초록 |
It is very important to accurately determine the concentration of H2O2 in body, which is parameter of activity coefficient in physiological reactions. Therefore, in this study, electrochemical biosensor based on hemoglobin (Hb)/DNA conjugate on the nanoporous Au electrode was developed to detect H2O2 with high sensitivity. The nanoporous Au electrode was designed to increase surface area of the electrode. The electrochemical biosensor was fabricated by hybridization of Hb/DNA conjugate and complementary DNA (cDNA) which immobilized on the nanoporous Au electrode. Since cDNA was firstly immobilized on the nanoporous Au electrode, Hb/DNA conjugate which introduced later could be uniformly arranged on the nanoporous Au electrode. Fabricated biosensor showed a wide linear range from 250 nM to 5.00 mM with a detection limit of 250 nM. Proposed biosensor could be utilized as a powerful sensing platform for development of electrochemical biosensor with high sensitivity. Acknowledgments: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government(MSIT) (No.2019R1A2C3002300) and by the Ministry of Education (No.2016R1A6A1A03012845). |
