| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.388 |
| 발표분야 |
생물화공 |
| 제목 |
Novel NAD(P)H Biosensor using Fe2O3/carbon/CB Nanocomposite Electrode Based on a Mediator-Free Electrochemical Method |
| 초록 |
There are some advantages and disadvantages using a mediator in electrochemical redox reaction. With a mediator, only a low overpotential is required, but the structure is complex. Without a mediator, however, the structure is simple but requires a high overpotential. High overpotential is accompaned by a low sensitivity, undesired side reactions and the electrode fouling. The main drawback of amperometric biosensors is a high overpotential, more than +0.7V. This could usually be overcome by using a mediator so far. We present here a novel method to solve this problem using an electrode modified with Fe2O3/carbon/CB nano-composite based on a mediator-free electrochemical method. Applyingthe Fe2O3/carbon/CB nanocomposite electrode can result in a huge increase in the sensitivity. The anodic peak potentials of the electrode with and withoutNADH were 0.185V and 0.146V (vs Ag/Agcl; pH 7.0) respectively. Under optimal conditions, NADH could be detected ina linear range from 10μM tomore than2000μM and the limit of detection was 2.5μM (S/N=3). The sensitivity was 494μA/mM (R2=0.997). |
| 저자 |
이정란1, 김양희1, 김지선1, 류지헌2, 오승모1, 유영제1
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| 소속 |
1서울대, 2한국산업기술대 |
| 키워드 |
NAD(P)H; biosensor; Fe2O3; nanocomposite; mediator-free; electrochemical method
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| E-Mail |
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| 원문파일 |
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