| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Drug-Delivery Nano-carrier based on Nitrilotriacetic Acid-End-Functionalized Poly(ε-caprolactone) |
| 초록 |
Polymer-protein core-shell hybrid structures are in the limelight as the new generation of nanocarriers due to the biocompatibility of proteins and the versatile functionalities of proteins. Here, we developed the protein-polymer nano-carriers by using nickel-complexed nitrilotriacetic acid-end-functionalized polycaprolactone (Ni-NTA-PCL) to apply drug-delivery system. Ni-NTA-PCL was prepared using a initiator containing NTA moiety via ring-opening polymerization. The GFP/PCL nano-carriers loaded with doxorubicin (DOX) (GFP/PCL-DOX) were constructed by adding DMF solution containing Ni2+-NTA-PCL and DOX to a phosphate buffer solution containing His6-GFP. DOX and PCL aggregated in water due to hydrophobic effects and His6-GFP was conjugated to the PCL particle by complexation between the His tag and Ni-NTA. The potential of GFP/PCL-DOX as drug vehicle was verified by the cellular uptake and cytotoxicity test on neuroblastoma SH-SY5Y cell. |
| 저자 |
황아란1, 이채연1, leeja jose1, 심규환2, 안성수2, 백현종1
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| 소속 |
1부산대, 2가천대 |
| 키워드 |
protein-polymer hybrid; nano-carrier; end-functionalized polymer
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| E-Mail |
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