| 초록 |
As widely known before, immobilized enzymes have lots of advantages such as multiple usage, easy separation and improved stability. There are several immobilizing techniques, including covalent immobilization, encapsulation and adsorption. Among these methods, we specifically studied encapsulation method using sol-gel technique in this research. Sol-gel methods is biologically inert and can be processed under room temperature and pressure, and the pore size of the matrices can be controled by changing sol conditions. And also its wide-range precursors make it possible be fabricated in many applications. So far, enzyme encapsulated sol-gel matrices for biocatalyst are used as a crushed powder form after xero-gel state. However, to be dried xero-gel state could cause the disruption of the pore and its 3-dimensional structures and moreover, the sol-gel particles followed by crushing process necessarily have irregular shapes and sizes. So in this study I focused on the process to develop the method to make the sol-gel particles in uniform-shaped and sized forms. |
