| 초록 |
We introduce self-assembled multi-lamellar complexes with the membrane leaflet changes of the cationic liposomes (DOTAP) mediated by anionic proteins, epidermal growth factor (EGF, pI = 4.6), in a layer-by-layer manner. The EGF/DOTAP complexes had the narrower size distribution than the DOTAP liposomes. There was no significant change of particle sizes at ambient temperature for 100 days. The loading efficiency of the EGF was above 95 %. FRET, cryo-TEM, Langmuir trough analyses, and molecular dynamic simulation were performed to understand the interactions of the EGF with the DOTAP liposomes. Acknowledgement: Basic Science Research Program (NRF-2013R1A1A1009626) |
