| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
우수논문(구두)_박사과정 |
| 제목 |
Evolutionary metabolic engineering of the 3-hydroxypropionic acid production pathway using a synthetic screening device |
| 초록 |
Evolutionary metabolic engineering has great progress for improving the metabolite-producing strains. In this case, it is important to develop an efficient screening method because of the low probability of incidence of positive mutants in a library. Therefore, in this study, a synthetic selection device using a transcription regulator was constructed and optimized for the efficient accomplishment of the evolutionary metabolic engineering. As the target metabolic pathway, the production pathway of 3-hydroxypropionic acid (3-HP), one of the important platform chemicals, has been adopted. The results indicate the successful applications to the 3-HP production pathway engineering, especially for enzyme engineering. Therefore, the strategy used in this study could expedite the strain improvement in the field of metabolic engineering by developing a high-throughput screening method. |
| 저자 |
석주연1, 양진아2, 최상진3, 최운종3, 유태현3, 정규열1
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| 소속 |
1포항공과대, 2서울대, 3아주대 |
| 키워드 |
evolutionary metabolic engineering; synthetic biology; directed evolution; 3-hydroxypropionic acid; selection; high-throughput screening
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| E-Mail |
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